DETERMINATION OF TOTAL PHENOLS, ANTHOCYANINS AND ANTIOXIDANT ACTIVITY IN THE BUTCHER’S BROOM (Ruscus aculeatus L) RIPE FRUIT

Gabidzashvili Manana; Guleishvili Nino; Inga Bochoidze

doi:10.31435/rsglobal_ws/30062023/8001

Gabidzashvili Manana PhD, Akaki Tsereteli State University, Kutaisi, Georgia
Guleishvili Nino PhD, Akaki Tsereteli State University, Kutaisi, Georgia
Inga Bochoidze Professor, Akaki Tsereteli State University, Kutaisi, Georgia

DOI: https://doi.org/10.31435/rsglobal_ws/30062023/8001

Keywords: The Butcher’s Broom Fruit; Total Phenols; Anthocyanins; Antioxidant Activity

Abstract

The useful properties of medicinal plants are due to the presence of various biologically active substances in them, such as phenolic compounds, alkaloids, anthocyanins, glycosides, saponins, essential oils, resins, mucus, vitamins and so on, which have a certain effect on physiological processes in the human body. The object of the study was the the butcher’s broom fruit growing wildly in Georgia, collected in August-September upon full maturation. Our research confirms that the alcoholic extract of the butcher’s broom fruit contains biologically active substances (total phenols - 39 , 4 mg/g; anthocyanins - 39.4 mg/g), which leads to the high antioxidant activity of the said extract - 45% (F=10).

References

Longo L, Vasapollo G. Determination of anthocyanins in Ruscus aculeatus L. berries. J Agric Food Chem 2005; 53: 475–479.

Mazza, G. Anthocyanins in fruits, vegetables and grains / G. Mazza, E. Miniati. - Boca Raton: CRC Press Inc; 1993. - 362 p.29.

Фракционный состав антоциановых красителей из растительных экстрактов и контроль над ними методом ВЭЖХ / О.Б. Рудаков [и др.] // Вестник ВГУ Серия: Химия. Биологя. Фармация. - 2004. - №1. - С. 85-93.

Аnthocyanins and other flavonoids / J.B. Harborne [et al.] // Natural Product Reports. – 2001. – Vol. 18. – Р. 310-333.).

Antioxidant activity of black currant anthocyanin aglycons and their glycosides measured by chemiluminescence in a neutral pH region and in human plasma / H. Matsumoto [et al.] // Journal of the Science of Food and Agriculture. – Vol. 50, N 18. – P. 5034-5037.

Kahkonen,M.P. & Heinonen,M. Antioxidant activity of anthocyanins and their aglycons. Journal of the Science of Food and Agriculture 51 (3), 628-633 (2003).

Hadzifejzovi ˇ c, ´ N., 2006. “Flavonoide und Hydroxyzimtsäureamide in Ruscus aculeatus L. und in anderen verwandten Arten”. Strukturaufklärung, Verteilung und physiologische Bedeutung. University of Münster, Germany (PhD Thesis).

Balica, G., Vostinaru, O., Tamas, M., Crisan G., Mogosan C. „Anti-inflammatory effect of the crude steroidal saponin from the rhizomes of Ruscus aculeatus L. (Ruscaceae) in two rat models of acute inflammation.“ J Food Agric Environ 2013; N11. Pp.106–108.

Bouskela, E., Cyrino, F.Z., Marcelon, G. „Inhibitory effect of the Ruscus extract and of the flavonoid hesperidine methylchalcone on increased microvascular permeability induced by various agents in the hamster cheek pouch“. J Cardiovasc Pharmacol 1993. N 22. Pp. 225–230.

Cappelli, R., Nicora, M., Di Perri, T. ,,Use of extract of Ruscus aculeatus in venous disease in the lower limbs“. https://pubmed.ncbi.nlm.nih.gov/3048951/.

Mimaki, Y., Kuroda, M., Kameyama A. ,,Steroidal saponins from the underground parts of Ruscus aculeatus and their cytostatic activity on HL-60 cells.” https://pubmed.ncbi.nlm.nih.gov/9654776/.

Gabidzashvili Manana, Guleishvili Nino, Inga Bochoidze Analysis Of Biologically Active Substances In Blueberry And Black Hawthorn Fruits Wild-Growing In Georgia “Scientifc Achievements of Modern Society”. Abstracts of V International Scientific and Practical Conference. Liverpool, United Kingdom. UDC 001.1 BBK 83. ISBN 978-92-9472-193-8].

Денисенко Т.А., Вишникин А.Б., Цыганок Л.П. Спектрофотометрическое определение суммы фенольных соединений в растительных объектах с использованием хлорида алюминия, 18-молибдодифосфата и реактива Фолина-Чокальтеу // Аналитика и контроль. 2015.T.19. №4. С.373-380. DOI:10.15826/analitika.2015.19.4.012.

О. В. Тринеева, А. И. Сливкин, М. А. Казьмина „Исследование спектральных характеристик антоциановых соединений плодов облепихи крушиновидной“ УДК 615.074 «Вестник МГОУ» 2014, № 3. 122. 5].