DEVELOPMENT OF THE SELECTIVE NUTRIENT MEDIUM FOR ISOLATION OF TOXIN PRODUCING STRAINS OF C. DIFFICILE

  • Said Kheder Молодший науковий співробітник, Державна установа «Інститут мікробіології та імунології ім. І. І. Мечникова Національної академії медичних наук України» https://orcid.org/0000-0002-0765-3157
Keywords: C. difficile, toxin-producing activity, nutrient media

Abstract

Enterocolitis disorders caused by Clostridioides difficile infection still remain a serious health problem in the world. In many countries CDI is officially considered a nosocomial infection that causes considerable economic losses, including diagnostic and treatment costs. According to the existing data, C. difficile is the main agent causing antibiotic – associated diarrhea and the main etiologic factor of the pseudomembranous colitis (PMC) that often develops in case of complete destruction of the intestinal flora due to the use of antibiotics or chemotherapeutic agents. There is no official registration of CDI in Ukraine, therefore the official incidence and lethality rates are absent.
At this time, the problems of development and improvement of selective nutrient mediums and quick, affordable bacteriological methods of C. difficile isolation are especially relevant.
The comparative study of the efficacy of the known commercial nutrient mediums for isolation of toxin-producing strains of C. difficile was carried out and composition of a new, original selective nutrient medium was proposed. Unlike existing analogs, the proposed nutrient medium is suitable for the simultaneous isolation of the agent from the clinical material and detection of toxin-producing properties due to its high growth properties, optimal transparency and density.

References

CDC. Antibiotic Resistance Threats in the United States, 2019. Atlanta, GA: U.S. Department of Health and Human Services, CDC. (2019). Retrieved from www.cdc.gov/DrugResistance/Biggest-Threats.html

Demihovskaya E. Dvulikiy C. difficile: vozbuditel ezhegodnoy infektsii i ili endogennyiy opportunist kishechnoy mikrofloryi pri antibiotikoterapii. «Bolezni i antibiotiki». № 1(03). 2010. Retrieved from http://www.mif-ua.com/archive/article/14568

Clostridium difficile. Robert Koch-Institut: Erstveröffentlichung im Epidemiologischen Bulletin Juni 2009. Retrieved from https://www.rki.de/

Cai J, Zhao C, Du Y, Zhang Y, Zhao M, Zhao Q. Comparative efficacy and tolerability of probiotics for antibiotic-associated diarrhea: Systematic review with network meta-analysis. United European Gastroenterol J. 2018 Mar;6(2):169-180. Retrieved from https://doi.org/10.1177/2050640617736987

Schäffler H, Breitrück A. Clostridium difficile - From Colonization to Infection. Front Microbiol. 2018 Apr 10; 9:646. Retrieved from https://doi.org/10.3389/fmicb.2018.00646

Lessa FC, Mu Y, Bamberg WM, Beldavs ZG, Dumyati GK, Dunn JR, et al. Burden of Clostridium difficile infection in the United States. N Engl J Med. 2015;372(9):825–34. DOI: 10.1056/nejmoa1408913

Dai, W., Yang, T., Yan, L. et al. Characteristics of Clostridium difficile isolates and the burden of hospital-acquired Clostridium difficile infection in a tertiary teaching hospital in Chongqing, Southwest China. BMC Infect Dis 20, 277 (2020). Retrieved from https://doi.org/10.1186/s12879-020-05014-6

Oren, Aharon; Garrity, George M. (2017). "List of new names and new combinations previously effectively, but not validly, published". International Journal of Systematic and Evolutionary Microbiology. 67 (9): 3140–3143. DOI:10.1099/ijsem.0.002278. PMC 5817221. PMID 28891789.

Lawson, Paul A.; Citron, Diane M.; Tyrrell, Kerin L.; Finegold, Sydney M. (August 2016). "Reclassification of Clostridium difficile as Clostridioides difficile (Hall and O'Toole 1935) Prévot 1938". Anaerobe. 40: 95–99. DOI:

1016/j.anaerobe.2016.06.008. ISSN 1095-8274. PMID 27370902. 10. Lobzin Y.V., Kvetnaya A.S., Zhelezova L.I. PEDIATRIC RESEARCH AND CLINICAL CENTER FOR INFECTIOUS DISEASES, ST. PETERSBURG, RUSSIA. Marine Medicine. 2017;3(1):14-24. (In Russ.) Retrieved from https://doi.org/10.22328/2413-5747-2017-3-1-14-24

Solovey NV, Karpov IA, Glaz OC (2013). C. difficile as the primary causative agent of antibiotic-associated diarrhea: current state of the problem [C. difficile kak osnovnoj vozbuditel’ antibiotik-assotsiirovannykh diarej: sovremennoe sostoyanie problemy]. Klinicheskaya infektologiya i parazitologiya. Prilozhenie, 102-125.

Waligora, A. J., Hennequin, C., Mullany, P., Bourlioux, P., Collignon, A., & Karjalainen, T. (2001). Characterization of a cell surface protein of Clostridium difficile with adhesive properties. Infection and immunity, 69(4), 2144–2153. https://doi.org/10.1128/IAI.69.4.2144-2153.2001

Thomas Jank, Torsten Giesemann, Klaus Aktories, Rho-glucosylating Clostridium difficile toxins A and B: new insights into structure and function, Glycobiology, Volume 17, Issue 4, April 2007, Pages 15R–22R. Retrieved from https://doi.org/10.1093/glycob/cwm004

Schirmer J, Aktories K. Large clostridial cytotoxins: cellular biology of Rho/Ras-glucosylating toxins. Biochim Biophys Acta. 2004 Jul 6;1673(1-2):66-74. Retrieved from https://doi:10.1016/j.bbagen.2004.03.014. PMID: 15238250

Voth, D. E., & Ballard, J. D. (2005). Clostridium difficile toxins: mechanism of action and role in disease. Clinical microbiology reviews, 18(2), 247–263. Retrieved from https://doi.org/10.1128/CMR.18.2.247-263.2005

Fiorentini, C., Fabbri, A., Falzano, L., Fattorossi, A., Matarrese, P., Rivabene, R., & Donelli, G. (1998). Clostridium difficile toxin B induces apoptosis in intestinal cultured cells. Infection and immunity, 66(6), 2660–2665. Retrieved from https://doi.org/10.1128/IAI.66.6.2660-2665.1998

Carter GP, Chakravorty A, Pham Nguyen TA, Mileto S, Schreiber F, Li L, Howarth P, Clare S, Cunningham B, Sambol SP, Cheknis A, Figueroa I, Johnson S, Gerding D, Rood JI, Dougan G, Lawley TD, Lyras D. Defining the Roles of TcdA and TcdB in Localized Gastrointestinal Disease, Systemic Organ Damage, and the Host Response during Clostridium difficile Infections. mBio. 2015 Jun 2; 6(3): e00551. Doi: 10.1128/mBio.00551-15. PMID: 26037121; PMCID: PMC4453007.

Barth H, Aktories K, Popoff MR, Stiles BG. Binary bacterial toxins: biochemistry, biology, and applications of common Clostridium and Bacillus proteins. Microbiology and Molecular Biology Reviews: MMBR. 2004 Sep;68(3):373-402, table of contents. DOI: 10.1128/mmbr.68.3.373-402.2004.

Volyanskij Yu. L., Chernyavs'kij V. I., Biryukova S. V., Maryushchenko A. M. i dr. Patogennye klostridii. Mikrobiologicheskaya diagnostika klostridial'nyh infekcij. Metodicheskie rekomendacii dlya vrachej i internov po special'nosti «Bakteriologiya». Har'kov. 2013. 60 S

Published
2020-10-16
Citations
How to Cite
Kheder, S. (2020). DEVELOPMENT OF THE SELECTIVE NUTRIENT MEDIUM FOR ISOLATION OF TOXIN PRODUCING STRAINS OF C. DIFFICILE. World Science, (8(60). https://doi.org/10.31435/rsglobal_ws/31102020/7216
Section
Medicine
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