УЛЬТРАСТУКТУРНА ОРГАНІЗАЦІЯ ГЕМОМІКРОЦИРКУЛЯТОРНОГО РУСЛА ШКІРИ БІЛОГО ЩУРА НА РАННІХ ЕТАПАХ ПЕРЕБІГУ ЕКСПЕРИМЕНТАЛЬНОГО СТРЕПТОЗОТОЦИН- ІНДУКОВАНОГО ЦУКРОВОГО ДІАБЕТУ

Abstract

This article is presented information about electron microscopic characteristic the angioarchitectonics of hemomicrocirculatory white rat skin net in experimental streptozotocin-induced diabetes mellitus. Was used in the experiment 30 adult white male rats weighing 120-130 grams, which were maintained on a standard diet, had free access to food and water under normal conditions. The insulin-dependent form of type I diabetes mellitus was modeled by a single intraperitoneal injection of streptozotocin from Sigma at a rate of 7mg per 100g of body weight of the animal (prepared on 0.1M citrate buffer, pH = 4.5). The development of experimental diabetes mellitus during 4 weeks was monitored by observing an increase in blood glucose, which was measured by the glucose oxidase method. Studies were performed on rats with glucose levels of 12.00 mmol/l and above. The experiment was carried out in accordance with the provisions of the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes (Strasbourg, 1986), Council of Europe Directive 86/609 / EEC (1986). Rats were removed from the experiment by an overdose of intraperitoneal anesthesia using sodium thiopental (calculated at 25 mg / kg body weight of the animal). Applying the method of electron microscopy (the study and photographing of the material was carried out on an electron microscope EM-100 AK at an accelerating voltage of 75 kV and magnification 6000-8000 times). As a material for this examination, the skin intact from the internal surface of the thigh and the back of the white rats was used. The study showed that after 2 weeks of diabetes occure gradually and are progressing alterations of the skin's hemomicrocirculatory bed of white rats, which characterised mainly by small spasm of some vessels. The wall of the most vessels still had normal structure but revealed a narrowing of the capillaries due to the protrusion of the nuclear zone of the endothelial cells. After 4 weeks of the experimental streptozotocin-induced diabetes mellitus were already observed reconstruction practicaly all skin's microvessels of white rats. The lumen of the capillaries became irregular. The contours of the nuclei of endotheliocytes were elongated with an uniform homogen chromatyn, which is concentrated in lumps, thickened bazal membrane, observed proliferation of endothelial cells, which leads to narrowing of the lumen of blood vessels. At the end of the experiment, we observe the inclusion of compensatory properties of the organism - the wall of blood vessels of the hemomicrocirculatory bed of the skin thickens, while the lumen of the microvessels become narrows. The results of investigation can be used in the practice medicine for diagnosis and treatment of skin's diseases in diabetes mellitus.