COMPARATIVE ANALYSIS OF LIPID PEROXIDATION ACTIVITY IN SUSPENSIONS OF CRYOPRESERVED CORD BLOOD NUCLEAR CELLS UNDER EXPOSURE TO ANTIOXIDANTS - MEMBRANOPROTECTORS WITH DIFFERENT ACTION MECHANISMS

Tetiana Kalynychenko; Anoshyna Militina; Balan Valentyna; Parubets Lidiia; Yagovdik Maryna

doi:10.31435/rsglobal_sr/30122020/7297

Tetiana Kalynychenko Doctor of Medical Sciences, PhD, Senior Researcher, Head of Laboratory of Hemopoietic Cell Cryopreservation. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine http://orcid.org/0000-0002-4905-3256
Anoshyna Militina PhD, Senior Researcher, Leading Researcher, Head of Biochemistry Group. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine https://orcid.org/0000-0001-6001-8016
Balan Valentyna Researcher of Laboratory of Immunogenetics. State Institution “National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine https://orcid.org/0000-0001-9663-7898
Parubets Lidiia Researcher of Laboratory of Hemopoietic Cell Cryopreservation. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine https://orcid.org/0000-0003-3418-1316
Yagovdik Maryna PhD, Senior Researcher of Biochemistry Group. State Institution "Institute of Hematology and Transfusiology of the National Academy of Medical Sciences of Ukraine", Kiyv, Ukraine https://orcid.org/0000-0003-2642-9609

DOI: https://doi.org/10.31435/rsglobal_sr/30122020/7297

Keywords: cryopreservation, umbilical cord blood, nuclear cells, lipid peroxidation, antioxidants

Abstract

An in-depth study of the oxidative homeostasis state into cell suspensions that contain hematopoietic stem cells is one of the key points for understanding ways to improve technologies for long-term storage of this material. Compounds with antioxidant action are considered promising additional cryoprotectants. Intensification of lipid peroxidation processes is one of the main factors causing disturbances in the barrier properties of cell membranes. Comparative analysis of changes in lipid peroxidation parameters during the cryopreservation-deconservation cycle showed that antioxidants-membrane protectors with different mechanisms of action (B-complex vitamins; α-lipoic acid, thiazotic acid morpholinium salt, 2-ethyl-6-methyl-3-hydroxy pyridine succinate) have similar features of a positive effect on the oxidative status of umbilical cord blood nuclear cell suspensions during cryopreservation. However, 2-ethyl-6-methyl-3-hydroxy pyridine succinate has a statistically significant advantage over α-lipoic acid and thiazotic acid morpholinium salt in terms of the conjugate formation dynamics during phospholipid peroxidation, which can be associated with its direct antioxidant effect.

References

Ballen, K. (2017 Aug). Update on umbilical cord blood transplantation. F1000Res [Internet],6, 1556. Retrieved from: https://f1000research.com/articles/6-1556/v1.

Kalynychenko, T.O. (2017). Umbilical cord blood banking in the worldwide hematopoietic stem cell transplantation system: perspectives for Ukraine. Exp Oncol, 39(3), 164-170. Retrieved from: https://exp-oncology.com.ua/article/10105

Tatone, C., Di Emidio, G., Vento, M., Ciriminna, R., & Artini, P.G. (2010 Aug) Cryopreservation and oxidative stress in reproductive cells. Gynecol Endocrinol, 26(8), 563-567. doi: 10.3109/09513591003686395.

Kaur, R., Pramanik, K., & Sarangi, S.K. (2013) Cryopreservation-induced stress on long-term preserved articular cartilage. Hindawi Publishing Corp: ISRN Tissue Engineering [Internet], Article ID 973542(10 pages). Retrieved from: https://doi.org/10.1155/2013/973542.

Белоус, А.М., & Грищенко, В.И. (1994). Криобиология. Киев: Наукова думка, 430.

Владимиров, Ю.А. (2000). Биологические мембраны и незапрограммированная смерть клетки. Соросовский образовательный журнал. Биология [Інтернет], (9), 2-9. Retrieved from: http://window.Edu.ru/resourse/554/20554/files/0009_002.pdf.

Лущак, В.І., Багнюкова, Т.В., & Лужна, Л.І. (2006). Показники оксидативного стресу. 2. Пероксиди ліпідів. Укр біохім журн, 78(5), 113-119. Retrieved from: http://ubj.biochemistry.org.ua/images/stories/pdf/2006/UBJ_N6_2006/Lushchak_78_6.pdf

Kalynychenko, Т. A., Anoshyna, M. Yu., & Balan, V. V. (2017). Advantages of umbilical cord blood cryopreservation using an unit volume reduction optimized method. Hematology. Transfusiology. Eastern Europe, 3 (4), 734-743.

Volchegorsky, I.A., Nalimov, A.G., Yarovinsky, B.G., & Lifshitz, R.I. (1989) Comparison of different approaches to the definition of LPO products in heptane - isopropanol blood extracts. Questions of Medical Chemistry, 35 (1), 127-131.

Anoshyna, M. Yu., Kalynychenko, T. O., & Glukhen’ka, G.T. (2011). The estimation of lipid’s peroxidation in cryopreserved patterns of umbilical cord blood. Ukrainian J. Hematology and Transfusiology, 11 (3), 12-15

Petrie, A., & Sabin, C.; Leonova, V. P. (Ed.) (2015). Medical statistics at a glance. Moscow: GEHOTAR-Media, 216.

Калиниченко, Т.О., Аношина, М.Ю., Балан, В.В., Мінченко, Ж.М., & Глухенька, Г.Т. (2013). Антиоксидантний захист кріоконсервованої гемопоетичної тканини пуповинної крові. Збірник наукових праць співробітників НМАПО ім. П.Л. Шупика, 22(2), 282-287. Retrieved from: https://nmapo.edu.ua/zagruzka/zbornikNMAPO22_2.pdf

Halliwell, B. (2012 May). Free radicals and antioxidants: updating a personal view. Nutrition Reviews, 70(5), 257-265. doi: 10.1111/j.1753-4887.2012.00476.x

Губский, Ю.И. (2015). Смерть клетки: свободные радикалы, некроз, апоптоз: монография. Винница: Новая книга, 360.

Sadowska-Bartosz, I., Pączka, A., Mołoń, M., & Bartosz, G. (2013 Dec). Dimethyl sulfoxide induces oxidative stress in the yeast Saccharomyces cerevisiae. FEMS Yeast Res, 13(8), 820-830. doi: 10.1111/1567-1364.12091.

Kang, M.H., Das, J., Gurunathan, S., Park, H.W., Song, H., Park, C., & Kim, J.H. (2017 Oct). The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study. Theranostics, 7(19), 4735-4752. doi: 10.7150/thno.21662.

Оковитый, С.В., Гайворонский, В.В., Куликов, А.Н., & Шуленин, С.Н. (2009). Клиническая фармакология. Избранные лекции: учебное пособие [Интернет]. Москва: ГЭОТАР-Медиа, 608. Retrieved from: http://www.studmedlib.ru/book/ISBN9785970411360.html.

Couto, N., Wood, J., & Barber, J. (2016 June). The role of glutathione reductase and related enzymes on cellular redox homoeostasis network. Free Radical Biology and Medicine, 95, 27-42. Retrieved from: https://doi.org/10.1016/j.freeradbiomed.2016.02.028.